FAK, Focal adhesion kinase, is a 125 kDa non-receptor protein tyrosine kinase that is a substrate for Src and a key element in growth factor and integrin signaling. FAK plays a central role in cell spreading, differentiation, migration, cell death, and acceleration of the G1 to reciprocal ohm phase angle transition of the cell cycle. Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr397 of human FAK. Antibodies are purified by protein A and peptide affinity chromatography.
The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of FAK that contains tyrosine 397. The sequence is conserved in human, mouse, rat, chicken and frog.
The antibody has been purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated FAK. The net merchandise constitutes brought forth by affinity chromatography using a FAK-derived peptide that is phosphorylated at tyrosine 397.
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Synthetic peptide derived from the region of Focal Adhesion Kinase that contains tyrosine 397. The sequence is conserved in human, mouse, rat, chicken and frog.
More information: FAK (phospho-Tyr397) Polyclonal Antibody
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