Applications Immunoblotting and Immunostaining
Species Request Info
Reactivity Human, Mouse, Chicken, Frog, Drosophila
Description
phospho-Tyr397 Polyclonal Antibody to FAK, phospho tyrosine 397
Affinity Purified Antibody (Stored in PBS [w/o Mg2+ or Ca2+], pH 7.3, with 1.0 mg/mL BSA [IgG and protease free] as a carrier), Contains 0.05% sodium azide
Focal Adhesion Kinase (FAK) is a 125 kD non-receptor protein tyrosine kinase that was discovered as a substrate for Src, and is a key element of integrin signaling. FAK plays a central role in cell spreading, differentiation, migration, cell death and acceleration of the G1 to S phase transition of the cell cycle. Tyrosine 397 is the autophosphorylation site of FAK. The site binds Src family SH2 domains and the p85 subunit of PI3-Kinase.
The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of FAK that contains tyrosine 397. The sequence is conserved in human, mouse, rat, chicken and frog.
The antibody has been purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated FAK. The final product is generated by affinity chromatography using a FAK-derived peptide that is phosphorylated at tyrosine 397.
The antibody is effective in immunoblotting and immunostaining.
More about: FAK (phospho-Tyr397) Polyclonal Antibody sale
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