Applications Dot, ELISA, WB
Species Human
Reactivity Human, Mouse, Rat.
Specificity
Phosphorylation site-specific antibody selective for the phosphorylated isoforms of glycogen synthase kinase enzyme, GSK 3 alpha, a 51 kDa protein containing a phosphate on tyrosine 279, and GSK 3 beta, a 47 kDa protein containing a phosphate on tyrosine 216.
Immunogen
Synthetic peptide derived from the regions of GSK 3 alpha + beta protein that contain tyrosine 216/279.
Glycogen synthase kinase 3 (GSK3) is a proline directed serine threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase, a key enzyme in glycogen metabolism. Since then, it has been shown to be involved in the regulation of a diverse array of cellular functions, including protein synthesis, cell proliferation, cell differentiation, microtubule assembly/disassembly, and apoptosis. GSK3s substrate specificity is unique in that phosphorylation of substrate only occurs if a phosphoserine or phosphotyrosine is present four residues C terminal to the site of GSK phosphorylation. There exists two isoforms of GSK3, alpha and beta, and they show a high degree of amino acid homology. The two isoforms of GSK3 are strictly regulated via phosphorylation. Phosphorylation of GSK3 beta on Ser9 (Ser21 in GSK3 alpha) by protein kinase B (PKB) causes its inactivation is the primary mechanism responsible for growth factor inhibition of this kinase. Activation of GSK3 beta is dependent upon the phosphorylation of Tyr216 (Tyr279 in GSK3 alpha). Upon activation, it has been shown to phosphorylate a number of different cellular proteins, including p53, c-Myc, c-Jun, heat shock factor 1 (HSF1), and cyclin D1. GSK3 beta also has been shown to phosphorylate aberrant sites on the microtubule associated protein tau, which is critical for the progression of Alzheimer's disease. GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation.
More about: GSK-3 alpha/beta (phospho-Tyr216/Tyr279) Polyclonal Antibody sale
Read more: phospho antibody
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